| Molecular Formula | C10H16N5O14P3 |
| Molar Mass | 523.18 |
| Density | 2.75±0.1 g/cm3(Predicted) |
| pKa | pK3:3.0(－2);pk5:7.10(－3);pK6:9.3(－4) (25°C,μ=0.1) |
| Physical and Chemical Properties | Chemical quality This product is white or white-like powder, odorless and hygroscopic. Soluble in water, insoluble in alcohol and chloroform. |
| Use | Use for the adjuvant treatment of chronic hepatitis, progressive muscular atrophy, vision loss and other diseases. |
| Raw Materials | Sodium hydroxide Diethyl ether Celite Potassium Phosphate Monobasic Pectin Anion exchange resin,strong basic quarternary ammoniumⅠ Adenosine triphosphate UTP CTP |
GMP-based fermentation method
Fermentation, gum removal, adsorption of potassium dihydrogen phosphate 12.5kg, magnesium chloride 1.2kg, glucose 2.5kg, yeast 170kg. Keep the yeast at 28-30 ℃, adjust pH = 7 with sodium hydroxide, ferment for 4h, the yeast starts to precipitate, filter to remove the yeast, and obtain enzyme solution. GMP is dissolved in water into an 8%-10% solution, 9 times the enzyme solution is added, fermented at 28-30 ℃ and pH 7 for about 3 hours, filtered at the end of the reaction, 2% diatomite is added to the filtrate, stirred and filtered, the filtrate is diluted with water to a GTP concentration of 0.3%, and adsorbed by strong alkaline quaternary ammonium I anion exchange resin column (717).
CMP [yeast] & rarr;[pH7,28-30 ℃] fermentation broth [diatomite] & rarr; Filtrate [717 resin] & rarr; Adsorbent
Elution and re-adsorption resin adsorbates are eluted with 0.04-0.07mol/L sodium chloride solution and 0.04-0.07mol/L hydrochloric acid, and the eluate is re-adsorbed with 717 resin.
Adsorbent [sodium chloride, hydrochloric acid] & rarr; Eluate [717 resin] & rarr; Adsorbent
Elution, decolorization, precipitation and drying of the secondary resin adsorbent are first eluted with 0.04-0.07mol/L of sodium chloride and 0.01mol/L of hydrochloric acid, then eluted with 0.5-1mol/L of sodium chloride solution, the eluate is added with a small amount of activated carbon and diatomite, stirred for 40min, filtered, the filtrate is added with 3 times of ethanol, let stand, filtered, the filter cake is washed with anhydrous ethanol and anhydrous ether, and the finished product of sodium guanosine triphosphate is obtained by vacuum drying.
Adsorbate [sodium chloride] & rarr; eluent [activated carbon, diatomite] & rarr;[40min] eluent [ethanol] & rarr;GTP [anhydrous ethanol, anhydrous ether] & rarr;GTP-Na.